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2-Nitrophenyl b-D-galactopyranoside
| CAS No: | 369-07-3 |
| Synonyms: ONPG |
| Product Code: | EN06363 |
| MDL No: | MFCD00063255 |
| Chemical Formula: | |
| Molecular Weight: | 301.25 |
| Product Structure
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| References: 1. Maruhn D, Clin. Chim. Acta 1976, 73, 453
2. Levvy GA, Conchie J, Methods Enzymol. 1966, 8, 571-84
3. Naider F, et al., Biochemistry 1972, 11, 3202-7 |
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| Pack Size | Pack Price | Hazardous Surcharge | Special Packaging | Number of Packs |
| 10g | $40.00 £26.00 €33.20 | Not Applicable Not Applicable Not Applicable | Not Applicable Not Applicable Not Applicable | |
| 25g | $75.00 £48.75 €62.25 | Not Applicable Not Applicable Not Applicable | Not Applicable Not Applicable Not Applicable | |
| 50g | $100.00 £65.00 €83.00 | Not Applicable Not Applicable Not Applicable | Not Applicable Not Applicable Not Applicable | |
100g 100g 100g | $150.00 £98.00 €125.00 | Not Applicable Not Applicable Not Applicable | Not Applicable Not Applicable Not Applicable | |
250g 250g 250g | $300.00 £195.00 €250.00 | Not Applicable Not Applicable Not Applicable | Not Applicable Not Applicable Not Applicable | |
Custom Pack  |
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Scale Available 100’s kgs
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ONPG
ONPG is the common abbreviation for (ortho) 2-Nitrophenyl-β-D-galactopyranoside.
ONPG is used in colourimetric and spectrophotometric assays for the detection of β-galactosidase activity which are of interest in clinical, environmental, food and molecular biology arenas.1 ONPG is hydrolysed by β-galactosidase to yield galactose and ortho-Nitrophenol, which is yellow in colour and absorbs light at 420 nm. The intensity of the colour can be used to determine the rate at which β-galactosidase can hydrolyse lactose. The impact of experimental factors on the reproducibility of β-galactosidase assays has been reported.2
In common with several other β-galactosidase substrates such as X-Gal, ONPG does not act as an inducer of genes under the control of the lac operon in bacteria such as E. coli. In the absence of a lactose analogue such as IPTG (Product Code: E10593 I) transcription of β-galactosidase will not take place and ONPG will not be hydrolysed.
Refs:
1. J Lederberg, J. Bacteriol., 1950, 20, 137.
2. A Giacomini et al, FEMS Microbiol. Lett., 2006, 100, 87.