In research published as a ‘Hot Paper’ in Angewandte Chemie International Edition in June 2019, Biosynth Carbosynth have developed novel chemiluminescent probes for the detection of foodborne pathogens in collaboration with scientists from the University of Tel Aviv, Zurich University of Applied Sciences and Nemis Technologies AG.
Two novel chemiluminescent probes known as CLSP and CLLP for detection of live Salmonella and Listeria monocytogenes bacteria have been characterised. These new probes are highly suitable for sensitive luminogenic bacterial enzymatic assays and offer a number of advantages:
- Ultrasensitivity – the improved luminophore has superior light emission and is 600-fold more sensitive than comparable fluorescent probes.
- A simple and quick experimental setup – enrichment and detection can be performed in a single test tube.
- Specificity – they are specific for pathogenic strains of Salmonella and Listeria monocytogenes.
The two substrates are prepared by masking a luminophore with specific substrates for the two pathogenic bacteria. A C8-ester group is used in the CLSP probe as a triggering group as it is a substrate for the Salmonella C8-esterase. In CLLP, a myo-inositol 1-phosphate group is conjugated to the luminophore as it is a substrate of the L. monocytogenes enzyme and virulence factor phosphatidylinositol phospholipase.
These two products complement our range of ultrasensitive AquaSpark™ chemiluminescent probes, which are available from Biosynth Carbosynth as enzyme substrates in 7 different formulations. CLSP is already available for purchase under the name AquaSpark™ caprylate.
Our AquaSpark™ chemiluminescent probes are making their way into diagnostic kits for rapid pathogenic bacteria detection with NEMIS Technologies, a joint venture formed by Biosynth and Ramot at Tel Aviv University. NEMIS Technologies is developing novel diagnostic solutions which will bring major improvements to microbiological testing assays in food safety, water treatment and clinical applications.
We are proud to be bringing innovation in the area of molecular biology as well as microbiological testing by developing improved molecules for ultrasensitive chemiluminescent detection.
For more information on the latest generation chemiluminescent probes, please look at our website or contact our technical experts.