5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt monohydrate
Chromogenic substrate for beta-glucuronidase (GUS) yieldinga blue precipitate. It is used for detection of GUS+ bacterial colonies and has been reported as chromogenic substrate in a number of chromogenic E. coli / coliform culture media.About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products, shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants.