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EI05931

Isopropyl b-D-thiogalactopyranoside - non-animal origin

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Technical Data

CAS No: 367-93-1
Synonyms: IPTG non-animal origin; IPTG
Product Code: EI05931
MDL No: MFCD00063273
Chemical Formula: C9H18O5S
Molecular Weight: 238.30

Literature


  • Barco A and Carrasco L (1992). Cloning and inducible synthesis of poliovirus non-structural proteins in Saccharomyces cerevisiae. Gene 156(1):19-25., Lee SG, et al (1988). Modulation of expression of the human gamma interferon gene in E.coli by site-directed mutagenesis. Biochem Biophys Res Commun 151: 598-607.
  • Date T, et al (1988). Expression of active rat DNA polymerase .beta. in Escherichia coli. Biochemistry 27(8): 2983–2990., Rodriguez JF and Smith GL (1990). IPTG-dependent vaccinia virus: identification of a virus protein enabling virion envelopment by Golgi membrane and egress. Nucleic Acids Res 18(18):5347-51.
  • Goeddel DV, et al (1979). Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A 76(1):106-10., Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press.
  • Hansen LH, et al (1998). The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Curr Microbiol 36(6):341-7., Zhang YF and Moss B (1991). Vaccinia virus morphogenesis is interrupted when expression of the gene encoding an 11-kilodalton phosphorylated protein is prevented by the Escherichia coli lac repressor. J Virol 65(11): 6101–6110.
  • Itakura K, et al (1977). Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science 198(4321):1056-63.

Solubility

Stability

Further Information


Datasheets

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Technical Bulletin: View

Isopropyl b-D-thiogalactopyranoside - non-animal origin

CAS No:
367-93-1
Synonyms:
IPTG non-animal origin
IPTG
MDL No:
MFCD00063273
Chemical Formula:
Molecular Weight:
238.30
Product Structure
References:
1. Barco A and Carrasco L (1992). Cloning and inducible synthesis of poliovirus non-structural proteins in Saccharomyces cerevisiae. Gene 156(1):19-25., Lee SG, et al (1988). Modulation of expression of the human gamma interferon gene in E.coli by site-directed mutagenesis. Biochem Biophys Res Commun 151: 598-607.
2. Date T, et al (1988). Expression of active rat DNA polymerase .beta. in Escherichia coli. Biochemistry 27(8): 2983–2990., Rodriguez JF and Smith GL (1990). IPTG-dependent vaccinia virus: identification of a virus protein enabling virion envelopment by Golgi membrane and egress. Nucleic Acids Res 18(18):5347-51.
3. Goeddel DV, et al (1979). Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A 76(1):106-10., Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press.
4. Hansen LH, et al (1998). The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Curr Microbiol 36(6):341-7., Zhang YF and Moss B (1991). Vaccinia virus morphogenesis is interrupted when expression of the gene encoding an 11-kilodalton phosphorylated protein is prevented by the Escherichia coli lac repressor. J Virol 65(11): 6101–6110.
5. Itakura K, et al (1977). Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science 198(4321):1056-63.
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