Coelenterazine is a bio-luminescent substrate of luciferases such as Renilla luciferase (Rluc) and Gaussia luciferase (Gluc). The mainapplications of Coelenterazine are monitoring of reporter genes andchemiluminescence-based methods for Calcium detection. The substrate is also used in bioluminescence resonance energy transfer (BRET), ELISA,HTS and chemiluminescent detection of superoxide anion and peroxynitritein cells or tissues. About this grade Coelenterazine, native, dried (C-7002): In order to provide milligram and sub-milligram quantities of native Coelenterazinewe pre-dissolve the substance; defined amounts of Coelenterazine aresubsequently dried under nitrogen in individual vials. Coelenterazine,native, dried (C-7002) is identical in quality and chemistry to ourproduct Coelenterazine, native (C-7001) which is provided as a powder. Information to the user: Since Coelenterazine is sensitive to oxygen,degas your solvents for stock solutions, if possible. To dissolve Coelenterazine, native, dried please proceed as follows. 1. Add the appropriate amount of solvent. If possible through the septum using a needle. Otherwise open the cap with pliers.2. Vortex the closed vial briefly to completely dissolve the content of the vial.3. Take out the solution, if possible with a needle through the septum.