Digitonin, stable clear, water soluble (D-3203) is a superior watersoluble Digitonin with improved stability for aqueous solutions. Digitonin is produced from seeds of Digitalis pupurea and other Digitalis species. It is a steroid glycoside consisting of the aglycondigitogenin linked to five sugar residues. Digitonin reacts hemolyticand is toxic to humans. Digitonin is frequently used for the solubilization of membrane proteins and for the permeabilization of cellular and nuclear membranes. Digitonin permeabilizes plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated β-hydroxysterols. This mechanism is used in procedures for the determination of cholesterol and ergosterol using Digiton. About this product: Aqueous solutions of conventional Digitonin will precipitate upon cooling to 4°C and will lose most of the biochemical function. Therefore, the precipitate is usually removed by filtration in many protocols. This filtration however decreases the concentration of Digitonin and alters the solubilizing power of the original product. In addition, precipitation and subsequent filtration result in an unknown Digitonin concentration in the final solutions and may negatively affect the reproducibility of any experiment with such solutions. In contrast to conventional Digitonin, our product Digitonin, stable clear, watersoluble (D-3203) is a special grade that will not precipitate for at least one week.